Conjugation and transposition in escherichia coli
Of escherichia coli the following account is based largely on this information it is important to note that the general features it is important to note that the general features. An erratum was issued for: creation of a dense transposon insertion library using bacterial conjugation in enterobacterial strains such as escherichia coli or shigella flexneri the discussion and references sections have been corrected. Bacterial conjugation was discovered by nobel prize winners joshua lederberg and edward tatum they showed that the bacterium escherichia coli entered a sexual phase during which it could share genetic information. To assess plasmid mobility, conjugation with plasmid-encoded args and sodium azide-resistant j53 e coli as the recipient was performed the antimicrobial resistance profile of the transconjugant (tc) was determined.
Abstract enteroaggregative escherichia coli (eaec) heat-stable enterotoxin 1 (east1) was originally discovered in eaec but has also been associated with enterotoxigenic e coli (etec. Helper plasmid pevs 104 may be particularly advantageous for recipients, such as the enterobacteriaceae, that replicate cole1- based vectors or support transposition of is903, mu, tn7, or e coli is elements. New york conjugative transposition of tn916 from enterococcus faecalis and escherichia coli into clostridium perfringens peter kaufmann, yvan lehmann, and leo melle institut fur lebensmittelwissenschaften, labor fur lebensmittelmikrobiologie, eidgenossische technische hochschule, eth-zentrum, ch-8092 zurich, switzerland.
Rates of transposition in escherichia coli ana sousa1, catarina bourgard1, lindi m wahl1,2 and isabel gordo1 1instituto gulbenkian de cieˆncia, oeiras, portugal. Ref: maintenance of broad-host-range incompatibility group p and group q plasmids and transposition of tn5 in bartonella henselae following conjugal plasmid transfer from escherichia coli dehio, c & meyer, m (1997) j bacteriol 179, 538–540. Isecp1-mediated transposition of qnrb-like gene in escherichia coli vincent cattoir, 1patrice nordmann, jesus silva-sanchez,2 paula espinal,3 and laurent poirel1 service de bacte´riologie-virologie, inserm u914 “emerging resistance to antibiotics,” hoˆpital de biceˆtre.
A novel qnrb-like plasmid-mediated resistance determinant, qnrb19, was identified from an escherichia coli clinical isolate from colombia its gene was associated with an isecp1-like insertion element that did not act as a promoter for its expression. Fifty-one fluoroquinolone-resistant escherichia coli isolates recovered from pigs, workers and environmental samples in one pig farm were screened for 16s rrna methylase genes and qepa, a fluoroquinolone efflux pump gene, by pcr. 文章 wollman el, jacob f, hayes w (1956) conjugation and genetic recombination in escherichia coli k-12 cold spring harb symp quant biol 21: 141–162. The occurrence of a genetic transposition in a strain of escherichia susanne k dewitt and edward a adelberg3 department of bacteriology, university of california, berkeley, california. The mcr-1 gene has often been identified from escherichia coli strains recovered from pigs (3,5–8) more recently, the mcr-2 gene, which shares 768% nt identity with mcr-1, has been identified from a single e coli isolate recovered from a pig in belgium.
Abstract: the primary objective of the experiment was to measure plasmid transfer and transposition frequency of escherichia coli using selection and contraselection of various combinations of antibiotics. Pathogenomics of the virulence plasmids of escherichia coli abstract bacterial plasmids are self-replicating, extrachromosomal elements that are key agents of. Phytopathology 75:104-108 transposon tn5, which confers kanamycin resistance, was introduced into the genomes of pseudomonas syringae pv syringae ps9020 and p syringae pv phaseolicola pp7010 by conjugation with escherichia coli smio containing the suicide plasmid vector psup1011 approximately of over 4,000. 1 transmission of genetic variation: conjugation bacterial conjugationis genetic recombination in which there is a transfer of dna from a living donor bacterium to a recipient bacterium.
Abstract we previously demonstrated efficient transformation of the thermophile geobacillus kaustophilus hta426 using conjugative plasmid. Quantitation and purification of acquired plasmid dna coding for antimicrobial drug resistance j roy johnson jr abstract this study provides evidence of direct transfer of functional dna f rom species to species, specifically escherichia coli pgfpuv and enterobacter aerogenes an escherichia coli made pgfpuv invasive. Abstract mobilizable shuttle plasmids containing the origin-of-transfer (orit) region of plasmids f (incfi), colib-p9 (inci1), and rp4/rp1 (incpα) were constructed to test the ability of the cognate conjugation system to mediate gene transfer from escherichia coli to saccharomyces cerevisiae. Rates of transposition in escherichia coli ana sousa1, catarina bourgard1, lindi m wahl1,2, isabel gordo1 1instituto gulbenkian de ciência, oeiras, portugal.
Effects of recb, recc and recf mutations on tn10 transposition in escherichia coli siu him chan, arthur lau, victor lei, and jashua woo j exp microbiol immunol 2005. (1996) tn7 transposition as a probe of cis interactions between widely separated (190 kilobases apart) dna sites in the escherichia coli chromosome j. Conjugative transfer of mobilizable derivatives of the escherichia coli narrow-host-range plasmids pbr322, pbr325, pacyc177, and pacyc184 from e coli to species of the gram-positive genera corynebacterium and brevibacterium resulted in the integration of the plasmids into the genomes of the recipient bacteria, transconjugants appeared at.